General description

MB49 cells are derived from C57BL/Icrf-a’; mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture . Transformed cells transplanted into syngeneic mice were shown to generate carcinomas . While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed . This abnormality is a frequent early event in human bladder cancer. A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth . MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone, human chorionic gonadotrophin (hCG) . MB49 cells exhibit low to no expression of MHC-Class I and II molecules . However, upon exposure to IFN-, the expressions of MHC Class I and II are significantly upregulated .

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the 'Academic Use Agreement' as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

Each vial contains ≥ 1X106 viable cells.
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination